Biotechnology and Microbiology: Video Clips Demonstrating Basic Practical Techniques
-
We have to create a sterile field before we perform experiments on microorganisms, this is to ensure no contamination of apparatus by other microorganisms during the…
Aseptic techniques (English subtitles available)
0 個讚 192 次播放 -
Streak plate can isolate a single bacterium and the colony formed on the agar plate. This video introduces the flame-sterilisation of inoculating loop, the technique of…
The method of streak plate (English subtitles…
0 個讚 103 次播放 -
This video introduces the tools and techniques for performing spread plate and pour plate.
The methods of spread plate and pour plate…
0 個讚 120 次播放 -
When we need to estimate the amount of bacteria in a sample, we usually perform serial dilutions of the bacterial sample or broth culture sample before culturing. This…
Serial dilutions and plate count (English…
0 個讚 92 次播放 -
This video introduces the method of culturing bacteria, which includes the transfer of one single colony or a tiny amount of broth culture to a new agar plate or a fresh…
Culturing bacteria (English subtitles available)
0 個讚 81 次播放 -
This video introduces the method of preparing culture media and pouring agar plates, including autoclaving of agar solution, reagent bottles and Petri dishes. Besides…
Preparing culture media and pouring an agar plate…
0 個讚 119 次播放 -
This video introduces the collection of microbial samples from some environmental surfaces using swab sticks, as well as the way of swabbing in order to collect as many…
Collection of microbial samples from some…
0 個讚 35 次播放 -
After performing microbiological experiments, it is important to sterilise the used apparatus and unwanted cultures properly before disposal, so that the environment…
Procedures and safety precautions of…
0 個讚 56 次播放 -
Gram staining is a common method to stain and to differentiate bacteria into two main groups: Gram positive bacteria (when stained purple) and Gram negative bacteria…
Gram staining (English subtitles available)
0 個讚 92 次播放 -
Micropipettes are used to draw and transfer a small amount of liquid accurately. There are 3 steps in transferring solution using micropipettes, i.e. setting the volume,…
Using a micropipette (English subtitles available)
0 個讚 310 次播放 -
Gel electrophoresis is one of the most common techniques in biotechnology, which can separate DNA samples (e.g. PCR products) according to their molecular sizes under an…
Gel electrophoresis (English subtitles available)
1 個讚 225 次播放 -
This video introduces the methods of mixing the substances in a centrifuge tube (i.e. finger-flicking and wrist-flicking), as well as the use of benchtop centrifuges and…
Methods for mixing and centrifuging of substances…
1 個讚 60 次播放