We have to create a sterile field before we perform experiments on microorganisms, this is to ensure no contamination of apparatus by other microorganisms during the…
Streak plate can isolate a single bacterium and the colony formed on the agar plate. This video introduces the flame-sterilisation of inoculating loop, the technique of…
When we need to estimate the amount of bacteria in a sample, we usually perform serial dilutions of the bacterial sample or broth culture sample before culturing. This…
This video introduces the method of culturing bacteria, which includes the transfer of one single colony or a tiny amount of broth culture to a new agar plate or a fresh…
This video introduces the method of preparing culture media and pouring agar plates, including autoclaving of agar solution, reagent bottles and Petri dishes. Besides…
This video introduces the collection of microbial samples from some environmental surfaces using swab sticks, as well as the way of swabbing in order to collect as many…
After performing microbiological experiments, it is important to sterilise the used apparatus and unwanted cultures properly before disposal, so that the environment…
Gram staining is a common method to stain and to differentiate bacteria into two main groups: Gram positive bacteria (when stained purple) and Gram negative bacteria…
Micropipettes are used to draw and transfer a small amount of liquid accurately. There are 3 steps in transferring solution using micropipettes, i.e. setting the volume,…
Gel electrophoresis is one of the most common techniques in biotechnology, which can separate DNA samples (e.g. PCR products) according to their molecular sizes under an…
This video introduces the methods of mixing the substances in a centrifuge tube (i.e. finger-flicking and wrist-flicking), as well as the use of benchtop centrifuges and…
