搜尋標籤: "inoculating loop"

We have to create a sterile field before we perform experiments on microorganisms, this is to ensure no contamination of apparatus by other microorganisms during the operations. This video introduces…

Streak plate can isolate a single bacterium and the colony formed on the agar plate. This video introduces the flame-sterilisation of inoculating loop, the technique of performing streak plate, as…

This video introduces the method of culturing bacteria, which includes the transfer of one single colony or a tiny amount of broth culture to a new agar plate or a fresh broth using the aseptic…

Gram staining is a common method to stain and to differentiate bacteria into two main groups: Gram positive bacteria (when stained purple) and Gram negative bacteria (when stained pink). There are 4…

在進行微生物實驗之前，我們必須建立一個無菌區域；實驗過程中亦要確保各操作和工具不被細菌污染。本短片介紹無菌技術的細節，例如：如何建立無菌區域，如何消毒容器的開口、接種環及塗抺棒。

平板劃線能夠分離在瓊脂平板上的單一細菌及其形成的菌落。本短片介紹如何以火焰消毒接種環；在瓊脂平板上進行劃線的技巧；以及如何在劃線時及觀察菌落前避免培養基受到污染。

革蘭氏染色是一種常用來把細菌染色以分類的方法，染色後的細菌可分成兩大類：革蘭氏陽性細菌(被染成紫色的)及革蘭氏陰性細菌(被染成粉紅色的)。革蘭氏染色包括四個步驟，按次序是染色、固定染料顏色、脫色及複染，各步驟需用不同的化學劑和技巧。